Etibor TA, Vale-Costa S, Sridharan S, Brás D, Becher I, Mello V, Ferreira, F., Alenquer, M., Savitski, M. Amorim, MJ. Defining the basic rules for hardening influenza A virus liquid condensates. 2023,eLife 12:e85182. https://doi.org/10.7554/eLife.85182. Also in 2022, bioRxiv, 022.2008.2003.502602.https://doi.org/10.1101/2022.08.03.502602.
Vale-Costa S, Etibor TA., Brás D, Sousa AL, & Amorim MJ. ATG9A facilitates the biogenesis of influenza A virus liquid condensates near the ER by dissociating recycling vesicles from microtubules. 2022,bioRxiv, 2022.2012.2021.521536. https://doi.org/10.1101/2022.12.21.521536
Sebastião CS, Cogle A, Teixeira AD, Cândido AM, Tchoni C, Amorim MJ, Loureiro N, Parimbelli P, Penha-Gonçalves C, Demengeot J, Sacomboio E, Mendes M, Arrais M, Morais J, Vasconcelos JNd, Brito M, Clinical Features Related to Severity and Mortality among COVID-19 Patients in a Pre-Vaccine Period in Luanda, Angola. 2022, Trop. Med. Infect. Dis.7, 338. https://doi.org/10.3390/tropicalmed7110338.
Clerbaux LA, Mayasich SA, Munoz A, Soares H, Petrillo M, Albertini MC, Lanthier N, Grenga L, Amorim MJ. Gut as an alternative entry route for SARS-CoV-2: current evidence and uncertainties of productive enteric infection in COVID-19. 2022, Journal of Clinical Medicine, 11(19). https://doi.org/10.3390/jcm11195691
Jani RA, Cicco AD, Keren-Kaplan T, Vale-Costa S, Hamaoui SD, Hurbain I, Tsai FC, Dimarco M, Macé AS, Zhu Y, AmorimMJ, Bassereau P, Bonifacino JS, Subtil A, Marks MS, Lévy D, Raposo G & Delevoye C. PI4P and BLOC-1 remodel endosomal membranes into tubules. 2022, JCB, https://doi.org/10.1083/jcb.202110132. Also 2021, bioRxiv, 2021.10.21.465321. https://doi.org/10.1101/2021.10.21.465321.
Clerbaux LA, Fillipovska J, Munoz A, Petrillo M, Coecke S, Amorim MJ, Grenga L. Mechanisms leading to gut dysbiosis in COVID-19: current evidence and uncertainties based on putative adverse outcome pathways. 2022, Journal of Clinical Medicine, 2022, 11(18), 5400; https://doi.org/10.3390/jcm11185400
Alenquer M, Milheiro Silva T, Akpogheneta O, Ferreira F, Vale-Costa S, et al. (2022) Saliva molecular testing bypassing RNA extraction is suitable for monitoring and diagnosing SARS-CoV-2 infection in children. PLOS ONE 17(6): e0268388. https://doi.org/10.1371/journal.pone.0268388
Alenquer M, Ferreira F, Lousa D, Valério M, Medina-Lopes M, Bergman M-L, et al. (2021) Signatures in SARS-CoV-2 spike protein conferring escape to neutralizing antibodies. PLoS Pathog 17(8): e1009772. https://doi.org/10.1371/journal.ppat.1009772
Gonçalves J, Melro M, Alenquer M, Araújo C, Castro-Neves J, Charepe N, et al. Balance between maternal antiviral response and placental transfer of protection in gestational SARS-CoV-2 infection. 2022, medRxiv. 2022:2022.08.23.22279113. https://doi.org/10.1101/2022.08.23.22279113.
Goncalves, J.; Julianao, A.M.; Charepe, N.; Alenquer, M.; Athayde, D.; Ferreira, F.; Archer, M.; Amorim, M.J.; Serrano, F.; Soares, H. Non-neutralizing secretory IgA and T cells targeting SARS-CoV-2 spike protein are transferred to the breastmilk upon BNT162b2 vaccination, 2021, Cell Reports Medicine. 2(12):100468, https://doi.org/10.1016/j.xcrm.2021.100468. Also in medRxiv10.1101/2021.05.03.21256416. 2021, https://doi.org/10.1101/2021.05.03.21256416.
Gabriel, L.; Srinivasan, B.; Kuś, K.; Mata, J.F.; Amorim, M.J.; Jansen, L.E.T.; Athanasiadis, A. Enrichment of Zα domains at cytoplasmic stress granules is due to their innate ability to bind nucleic acids, 2021,J Cell Sci, jcs.258446. https://doi.org/10.1242/jcs.258446
Etibor, T.A.; Yamauchi, Y.; Amorim, M.J. Liquid Biomolecular Condensates and Viral Lifecycles: Review and Perspectives. Viruses, 2021, 13, 366. https://doi.org/10.3390/v13030366
Gabriel, L.; Srinivasan, B.; Kuś, K.; Mata, J.F.; Amorim, M.J.; Jansen, L.E.T.; Athanasiadis, A. Enrichment of Zα domains at cytoplasmic stress granules is due to their innate ability to bind nucleic acids. bioRxiv, 2021, 10.1101/2021.01.20.427402, 2021.2001.2020.427402, https://doi.org/10.1101/2021.01.20.427402
Ho, J S Y, Angel, M, Ma, Y, Sloan, E, Wang, G, Martinez-Romero, C, Alenquer, M, Roudko, V, Chung, L, Zheng, S, Chang, M, Fstkchyan, Y, Clohisey, S, Dinan, A M, Gibbs, J, Gifford, R, Shen, R, Gu, Q, Irigoyen, N, Campisi, L, Huang, C, Zhao, N, Jones, J D, van Knippenberg, I, Zhu, Z, Moshkina, N, Meyer, L, Noel, J, Peralta, Z, Rezelj, V, Kaake, R, Rosenberg, B, Wang, B, Wei, J, Paessler, S, Wise, H M, Johnson, J, Vannini, A, Amorim, M J, Baillie, J K, Miraldi, E R, Benner, C, Brierley, I, Digard, P, Łuksza, M, Firth, A E, Krogan, N, Greenbaum, B D, MacLeod, M K, van Bakel, H, Garcìa-Sastre, A, Yewdell, J W, Hutchinson, E, Marazzi, I, Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection, 2020, Cell. URL:https://doi.org/10.1016/j.cell.2020.05.035
Alenquer M, Vale-Costa S, Sousa AL, Etibor TA, Ferreira F and Amorim MJ, Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites, 2019, Nature Communications. 10, Article number: 1629 (2019) https://doi.org/10.1038/s41467-019-09549-4(Research article). See Press Release here.
Nieto A, Vasilijevic J, Santos NB, et al. Mutation S110L of H1N1 Influenza Virus Hemagglutinin: A Potent Determinant of Attenuation in the Mouse Model. Front Immunol. 2019;10:132. Published 2019 Feb 6. doi:10.3389/fimmu.2019.00132(Research article).
Amorim MJ. A Comprehensive Review on the Interaction Between the Host GTPase Rab11 and Influenza A Virus. Front Cell Dev Biol. 2019;6:176. Published 2019 Jan 9. doi:10.3389/fcell.2018.00176
Pereira CF, Wise HM, Kurian D, et al. Effects of mutations in the effector domain of influenza A virus NS1 protein. BMC Res Notes. 2018;11(1):673. Published 2018 Sep 18. doi:10.1186/s13104-018-3779-6(Research article).
Ramos-Nascimento A, Kellen B, Ferreira F, Alenquer M, Vale-Costa S, , Raposo G, Delevoye C and Amorim MJ, “KIF13A mediates influenza A virus ribonucleoproteins trafficking”, Journal of Cell Science, 2017, 130:4038-4050. doi: 10.1242/jcs.210807 (Research article).
Pereira CF, Read EKC, Wise HM, Amorim MJ and Digard P, “The influenza A virus NS1 protein promotes efficient nuclear export of unspliced viral M1 mRNA”, 2017, J. Virology, 91, pii: e00528-17. doi: 10.1128/JVI.00528-17 (Research article).
Sousa AL, Vale-Costa S, Amorim MJ and Tranfield EM, “Using correlative light and electron microscopy to understand influenza A viral assembly", Ultrastructural Pathology, 2017, 41:80. https://doi.org/10.1080/01913123.2016.1269508 (Research article).
Vale-Costa S, Alenquer M, Sousa AL, Kellen B, Ramalho J, Tranfield EM and Amorim MJ, “Influenza A virus ribonucleoproteins modulate host recycling by competing with Rab11 effectors”, Journal of Cell Science, 2016, 129: 1697-1710. https://doi.org/10.1242/jcs.188409 (Research article).
Vale-Costa S and Amorim MJ, “Clustering Rab11 vesicles in influenza A virus infected cells creates hotspots containing the 8 viral ribonucleoproteins”, Small GTPases, 2016, 23: 0-7. https://doi.org/10.1080/21541248.2016.1199190 (Review).
Vale-Costa S and Amorim MJ, “Recycling endosomes and Viral Infection”, Viruses, 2016, 8: 64-92. https://dx.doi.org/10.3390/v8030064 (Review).
Alenquer M, and Amorim MJ, “Exosomes in viral infection: More than ESCRT(ing) viral budding”, Viruses, 2015, 7: 5066-5083. https://dx.doi.org/10.3390/v7092862 (Review).
THE PI BEFORE BECOMING A PI:
Amorim MJ, Kao R and Digard P, “Nucleozin targets vRNP-Rab11 complexes circulating in the cytoplasm in influenza A virus infection”, J. Virology, 2013, 87:4694. https://dx.doi.org/10.1128/JVI.03123-12.
Amorim MJ‡, Bruce EA‡, Read EK, Foeglein A, Mahen R, Stuart AD and Digard P, “A Rab11 and microtubule dependent mechanism for transporting influenza A virus vRNA to the plasma membrane via the pericentriolar recycling endosome”, J. Virology, 2011, 85: 4143. https://dx.doi.org/10.1128/JVI.02606-10.
Amorim MJ and Mata, J. “Global coordination of transcriptional control and mRNA decay during cellular differentiation”, Molecular Systems Biology, 2010, 8: 380. https://dx.doi.org/10.1038/msb.2010.38
Amorim MJ and Mata, J, “Rng3p, a member of the UCS family of myosin co-chaperones, associates with myosin heavy chains cotranslationally”, EMBO reports, 2009, 10:186-91. https://dx.doi.org/10.1038/embor.2008.228
Amorim MJ, Read EK, Medcalf L and Digard P, “Nuclear export of Influenza A virus mRNAs requires ongoing RNA polymerase II activity”, Traffic, 2007, 8: 1. https://doi.org/10.1111/j.1600-0854.2006.00507.x
Dalton RM, Mullin AE, Amorim MJ, Medcalf L, Tiley L and Digard P, “Temperature sensitive influenza A virus genome replication results from low thermal stability of polymerase-cRNA, complexes”, Virology Journal, 2006, 25: 58. https://dx.doi.org/10.1186/1743-422X-3-58
Elton D, Amorim MJ, Medcalf L and Digard P, “ ‘Genome gating’; polarized intranuclear trafficking of influenza virus RNPs”, Biology Letters, 2004, 1: 113. https://dx.doi.org/10.1098/rsbl.2004.0253. See also Research in Polar Flu Highlight Section in Nature, 2004, 434: 1084. https://dx.doi.org/10.1038/4341084a
Mullin AE, Dalton RM, Amorim MJ, Elton D, Digard P, “Increased amounts of the influenza virus nucleoprotein do not promote higher levels of viral genome replication”, J Gen Virol., 2004, 85:3689. https://dx.doi.org/10.1099/vir.0.80518-0
Carrasco M, Amorim MJ, Digard P, “Lipid raft-dependent targeting of the influenza A virus nucleoprotein to the apical plasma membrane”, Traffic, 2004, 5:979.https://doi.org/10.1111/j.1600-0854.2004.00237.x
Amorim MJ, Ferreira JP, “Microparticles for drug delivering therapeutic peptides and proteins to the lumen of the small intestine”, Eur J Pharm Biopharm,2001, 52:38. https://dx.doi.org/10.1016/S0939-6411(01)00148-5
Hagen WR, Silva PJ, Amorim MA, Hagedoorn PL, Wassink H, Haaker H, Robb FT, “Novel structure and redox chemistry of the prosthetic groups of the iron-sulfur flavoprotein sulfide dehydrogenase from Pyrococcus furiosus; evidence for a [2Fe-2S] cluster with Asp(Cys)3 ligands”, J. Biol Inor Chem, 2000, 5: 527. https://doi.org/10.1007/PL00021452.
SELECTED CONFERENCE COMMUNICATIONS:
at the igc (BY ALL LAB MEMBERS)
Invited seminar on “Rules for hardening influenza A virus liquid inclusions”, Microbiology Society Annual Conference 2023, 17th-20th April 2023 [invited by Organizing committee of Microns apart: viral organization of the crowded cell].
Invited seminar on Viruses as Adverse Outcome Pathway Stressors, CIAO Project Final online Workshop, 8-9th February 2023, virtual [invited by Organizing committee].
Invited seminar on “Rules for hardening influenza A virus liquid inclusions”, Leibniz Center Infection Symposium 2023, 26th-27th January 2023, Hamburg, Germany [invited by Organizing committee].
Invited seminar on “Rules for hardening influenza A virus liquid inclusions”, Meeting PhasAge, 11th-13th December 2022, Brussels, BE [invited by Peter Tompa].
Speaker on “Exploring additional layers of complexity in influenza A virus host-interaction, NSV2022, 12th-17th June 2022, PT.
Invited seminar on “New paradigms in influenza A virus infection” iMM Monday Lecture series, Lisbon, Portugal, 12thJuly 2021. Invited moderator of session “Ciências da Saúde - Doenças Infeciosas e Combate a Agentes Patogénicos” for Portuguese Scientific Meeting 2021, Organized by the FCT, Lisbon, 30th June 2021
Invited speaker, JCR summer school 2021 “Neutralization assays for measuring how SARS-CoV2 variants escape immunity”, Ispra, Italy, 19th May 2021.
Invited speaker “The dialogue between membrane-bound and liquid membraneless organelles during influenza A vírus infection” Institute Curie, 2nd March 2021.
Invited speaker on “The impact of emerging SARS-CoV2 variants in vaccination strategies” webinar on COVID vaccines, 24th February 2021, Fundação Calouste Gulbenkian and La Caixa, virtual meeting.
Invited speaker “New paradigms in cell biology”, II symposium in applied biochemistry, 29th January 2021, University of Minho, PT.
Invited seminar “Invited seminar about SARS-CoV-2: Knowns, Unknowns and controversies”, Univerza v Ljubljani, 4thDecember 2020, invited by Emilija Stojmenova Duh.
Invited speaker “How pandemics change lives", Dpt of State of the United States, 28th October 2020
Invited speaker “Influenza A virus ribonucleoproteins form liquid organelles at Endoplasmic reticulum exit sites”, EMBO Proteostasis 2019 Meeting, 15th-18th November 2019, Ericeira, PT.(ATTENDEE: Amorim M.J.)
Invited speaker “Influenza A virus ribonucleoproteins form liquid organelles at Endoplasmic reticulum exit sites”, SPAOM 2019 Meeting, 6th-8th November 2019, Coimbra, PT. (ATTENDEE: Amorim M.J.)
Speaker: “IMAGING LIQUID ORGANELLES FORMED DURING INFLUENZA INFECTION”, Frontiers in Cellular, Viral and Molecular Microscopy 16th-17th September 2019, Bristol UK. (ATTENDEE: Amorim M.J.)
Speaker: Marta Alenquer, “Influenza a virus ribonucleoproteins form liquid organelles at Endoplasmic reticulum exit sites”, Influenza Update meeting, 17th-18th December 2018, University of Cambridge, UK [Contributed talk]
Speaker-Flash poster session:“Influenza A virus ribonucleoproteins form liquid organelles at Endoplasmic reticulum exit sites”, 'Cell Dynamics: Organelle-Cytoskeleton Interface' Meeting, 19th-22nd May 2019, JCS, Lisbon, PT.(ATTENDEE: Amorim M.J.)
Speaker: “Influenza A virus ribonucleoproteins form liquid organelles at Endoplasmic reticulum exit sites”, Influenza Update meeting, 17th-18th December 2018, University of Cambridge, UK.(ATTENDEE: Amorim M.J.)
Keynote speaker:“Strategies of influenza A virus adaptation to hosts”, TIBE 2018, international conference, CiBio, 7th-8th December 2018, PT [Invited by TIBE organizing committee, University of Porto, KEYNOTE TALK].(ATTENDEE: Amorim M.J.)
Speaker,Amorim M.J. “Membrane regulators of complement activation modulate influenza A virus infection”, Munster, 6th International Influenza Meeting, 2-4th September 2018, Munster, Germany.(ATTENDEE: Amorim M.J.)
Invited speaker: “Mechanisms of influenza A virus modulation of host membrane trafficking”, external seminars, University of Aveiro, 9th May 2018, PT [Invited by Daniela Ribeiro, University of Aveiro, seminar].(ATTENDEE: Amorim M.J.)
Invited speaker: “Mechanisms of influenza A virus modulation of host membrane trafficking”, Cambridge Virology seminars, University of Cambridge, Dpt of Pathology/Virology, 26th April 2018, Cambridge, UK [Invited by Organiser committee, Division of Virology, University of Cambridge, seminar].(ATTENDEE: Amorim M.J.)
Invited speaker:“Mechanisms of influenza A virus assembly”, Meeting on Vesicular Biology, 16th-17th February 2018, Coimbra, PT [Invited by Organiser committee, Faculty of Medical Sciences, University of Coimbra, contributed talk].(ATTENDEE: Amorim M.J.)
Poster: “Membrane regulators of complement activation modulate influenza A virus infection”, Viruses 2018 - Breakthroughs in Viral Replication Conference, 7th-9th February 2018, Barcelona, Spain [Poster].(ATTENDEE: Amorim M.J.)
Speaker: “Membrane regulators of complement activation modulate influenza A virus infection”, Influenza Update meeting, 21st-22nd November 2017, University of Roslin, Edinburgh, UK.(ATTENDEE: Amorim M.J.)
Invited speaker: “Mechanisms of influenza A virus assembly”, BioSAM conference FCUL, 22nd April 2017, Lisbon, PT [Invited by Organiser committee, Faculty of Sciences, University of Lisbon, , contributed talk].
Poster: Santos N., Vaz da Silva Z.E. and Amorim M.J. “Influenza A virus-mediated alterations of complement decay-accelerating factor”, EMBO Workshop Proteostasis, Ericeira, Portugal, November 17-21th, 2017 (ATTENDEE: NSantos)
Poster: Vaz-da-Silva ZE and Amorim MJ, Modulation of DAF and CD59 by influenza A viruses, 27th Annual Meeting of the Society for Virology (GFV), Margurg, Germany, March 22-25th, 2017 (ATTENDEE: Zoé E. Vaz-da-Silva).
Talk: Membrane Regulators of Complement Activation impact in influenza A virus pathogenicity, Sociedade Portuguesa de Imunologia, Lisboa, Portugal, June 1-3rd, 2016 (ATTENDEE: Zoé E. Vaz-da-Silva).
Poster: Costa S., Alenquer M., Kellen B and Amorim MJ, “Influenza A virus infection “stalls” sorting of recycling endosomes”, Viruses and cells, Gordon Research Conferences, Barcelona, Spain, Jun 21-26th, 2015 (ATTENDEE: MJAmorim).
Poster: Vaz-da-Silva, EZ and Amorim MJ, “Deficiency in regulators of complement activation - DAF and CD59 - protects against influenza A virus infection”, Keystone Symposiaon Innate Immunity and Determinants of Microbial Pathogenesis, California, USA, April 19-24th, 2015 (ATTENDEE: Zoé E. Vaz-da-Silva)
Poster:Costa S., Alenquer M., Kellen B and Amorim MJ, “Alterations to the recycling endosome during influenza A virus infection”, 33rd American Society of Virology Conference, Colorado, USA, Jun 21-26th, 2014 (ATTENDEE: Silvia Vale-Costa)
Talk:Amorim MJ, Kao R, Digard P., “Influenza A virus replication can be prevented by inhibition of vRNP transport”, 32nd ASV Meeting, Penn State University, USA, July 20-24th, 2013 (ATTENDEE: MJAmorim)
Talk: Amorim MJ, Kao R, Digard P., “Proof of principle for inhibition of vRNP transport in Influenza A virus infection”, 3rd Int. Influenza Meeting, Munster, Germany, 1-3 Sep 2012 (ATTENDEE: MJAmorim)
- 7th January 2022 – Participation in documentary - Viruses – obligatory parasites with three episodes exhibited in RTP1, Portuguese television (links: https://www.rtp.pt/play/p9768/e590721/virus;
- 2nd December 2021 - Participation in the documentary – “The art of curing people”, appearance in several episodes – recorded on 3rd of March 2020 and broadcasted from the 2nd of December in RTP2 – Rita Saldanha. https://www.rtp.pt/play/p9539/e597918/a-arte-da-cura
July 2019 – Commercial on dynamics and innovation by Journal Observador, sponsored by Seat.
- May 2019 – Invited for TV programme “consultório” broadcasted by Porto Canal for a 30 minute interview on the importance of fundamental research in virology - http://portocanal.sapo.pt/um_video/QdnHyEHYQZZv0zmdIFLR - May 2019 – Invited for TV news programme “Edição da manhã” by the TV channel SIC.
- April 2019 - Invited for TV news programme “Diário da manhã” by the TV channel TVI, broadcasted simultaneously in TVI24.
- Feb 2019 – Invited as scientist to discuss students perspectives and careers “Jornadas Tecnológicas de Biologia (JORTEC)”, FCT, Universidade Nova, PT.
- May 2018 - Invited seminar on “Vaccination - myths and the science behind it”, for the immunology day organized by the IGC. [Invited by IGC Communication Team, PT].
- Invited lecture on “Yearly epidemics and occasional pandemics”, for the program Schools organized by the IGC, March 2016 (MJAmorim).
- INVITED speaker, ENEB, University of Braga, March 2015 (MJAmorim)
- INVITED speaker, for "Ciència a 4 tempos", Faro, March, 2015
- INVITED TEXT for newspaper entitled "what have viruses taught us about cancer?", June 2015 (MJAmorim)
- Invited speaker, Science Summerschool ANEM 2015, “Motivation to do science (for medical doctors)”, ITQB, July 2015 (MJAmorim). - Invited speaker in Greenfest, Estoril, to discuss “Flu epidemics and pandemics”, Portugal, October 2015 (MJAmorim).
- Organization of the activity on awareness on "Transmission of influenza A virus", IGC Open Day, Oct 2014 (all lab members).
Check Radio interview on this website: part 1: https://soundcloud.com/ciencia_viva_conversa/maria-joao-amorim-virologista-1-8-abr-2015 part 2: http://www.sulinformacao.pt/2015/04/maria-joao-amorim-virologista-2-15-abril-2015/